Rem2 interacts with CaMKII at synapses and restricts long-term potentiation in hippocampus

Synaptic plasticity, the process whereby neuronal connections are either strengthened or weakened in response to stereotyped forms of stimulation, is widely believed to represent the molecular mechanism that underlies learning and memory. The holoenzyme calcium/calmodulin-dependent protein kinase II (CaMKII) plays a well-established and critical role in the induction of a variety of forms of synaptic plasticity such as long-term potentiation (LTP), long-term depression (LTD) and depotentiation. Previously, we identified the GTPase Rem2 as a potent, endogenous inhibitor of CaMKII. Here, we report that knock out of Rem2 enhances LTP at the Schaffer collateral to CA1 synapse in hippocampus, consistent with an inhibitory action of Rem2 on CaMKII in vivo. Further, re-expression of WT Rem2 rescues the enhanced LTP observed in slices obtained from Rem2 conditional knock out (cKO) mice, while expression of a mutant Rem2 construct that is unable to inhibit CaMKII in vitro fails to rescue increased LTP. In addition, we demonstrate that CaMKII and Rem2 interact in dendritic spines using a 2pFLIM-FRET approach. Taken together, our data lead us to propose that Rem2 serves as a brake on synaptic potentiation via inhibition of CaMKII activity. Further, the enhanced LTP phenotype we observe in Rem2 cKO slices reveals a previously unknown role for Rem2 in the negative regulation of CaMKII function.


Introduction
CaMKII is an abundant, multifunctional serine-threonine kinase whose regulation and activity are uniquely sensitive to synaptic activity [1].Decades of biochemical, genetic, and electrophysiological experiments established a critical role for CaMKII in the synaptic strengthening (i.e., long-term potentiation (LTP)) that underlies learning and memory in the hippocampus [2,3].CaMKII monomers contain an N-terminal catalytic domain, central regulatory domain, and a C-terminal association domain which mediate assembly into the dodecamer, holoenzyme form [4].In the canonical pathway for LTP induction at CA3-CA1 synapses of the hippocampus, high-frequency stimulation triggers the activation of N-methyl-D-aspartate receptors (NMDARs) [5].Subsequent Ca 2+ influx through the NMDAR ionophore activates calmodulin (CaM) which binds to the holoenzyme CaMKII causing a conformational change, displacing the regulatory domain and exposing the substrate binding site(s) and, notably, a threonine residue (T286) on the regulatory domain [6].Trans-autophosphorylation of the T286 site by an activated CaMKII catalytic domain results in an autonomously active form of CaMKII which allows its activity to persist even in the absence of Ca 2+ [7].LTP is expressed as an increase in AMPAR function: increased number of receptors [8] and increased single channel conductance [9].
Many studies point to a role for the catalytic activity of CaMKII in LTP expression via phosphorylation of downstream targets (e.g. the GluA1 subunit of the α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor (AMPAR)) subsequent to T286 phosphorylation [10][11][12][13].Manipulations that block the catalytic activity of CaMKII such as application of peptide inhibitors [2,14] or deletion of the CaMKIIα gene impair LTP [15].However, it is also known that CaMKII binding to the GluN2B subunit of the NMDAR [16,17] plays a critical role in LTP induction [15,18,19].In fact two recent studies demonstrate that the enzymatic function of CaMKII towards non-CaMKII substrates is dispensable for LTP induction while CaMKII binding to GluN2B, a "structural" function of CaMKII, is required [20,21].In addition, new X-ray crystallographic structures reveal that CaMKII substrates bind along one continuous binding site [22] as opposed to two distinct sites (i.e., S-and T-sites) on the catalytic domain [23].Because inhibitors of CaMKII catalytic activity which are known to impair LTP also block GluN2B binding [14] the critical role of CaMKII-GluN2B binding in LTP was obscure until now.
The GTPase Rem2 is a potent (K i ~6nM) inhibitor of CaMKII catalytic activity in vitro [24].Rem2 inhibits the autonomously active CaMKII holoenzyme, does not interfere with CaMKII autophosphorylation at T286, and does not interfere with calmodulin (CaM) binding [24].Based on these data we favor a model by which the N-terminus of Rem2, which contains a CaMKII pseudosubstrate sequence, inhibits CaMKII in vitro by binding to the substrate binding site in the CaMKII catalytic domain.In so doing, Rem2 may also block access of the GluN2B binding site on CaMKII.
Rem2 is a member of the RGK family of non-canonical Ras-like GTPases (which also includes Rad, Gem/Kir, and Rem) and is primarily expressed in the brain [25].A number of features differentiate RGK family members from the Ras superfamily.For example, the crystal structures of several RGK proteins, including Rem2, reveal differences between this family and classical GTPases in the structure of their nucleotide-binding domains [26,27].With the exception of the identification of NmeI as a Rad GTPase activating protein (GAP) [28], neither GAPs nor guanine nucleotide exchange factors (GEFs) have been identified for this protein family [29].For these and other reasons, Rem2 and the RGK family members in general may not behave as classical Ras-like GTPases regulated by their nucleotide-binding state [30].
Overexpression of RGK family members in a variety of cell types results in cytoskeleton rearrangements and decreased Ca 2+ influx through voltage-gated calcium channels [31][32][33][34][35] suggesting that RGK proteins are multifunctional signal transducers.However, the signaling mechanism(s) by which Rem2 and other RGK proteins mediate these processes are not well understood.Previous studies from our lab using Rem2 knockout approaches demonstrated that Rem2 is a critical regulator of dendritic branching, synapse formation and cortical plasticity in the vertebrate nervous system [35][36][37][38][39][40][41].In addition, Rem2 and CaMKII co-associate in neurons [24,42] and interact in a signal transduction pathway that regulates dendritic arborization [24,37,38].
Here, we test the hypothesis that Rem2 plays a role in CaMKII-dependent LTP.We posited that Rem2 could influence synaptic plasticity through an interaction with CaMKII in the postsynaptic dendritic spine, the site of CaMKII activation and LTP induction and expression.To test this hypothesis, we studied the expression of Rem2 in the hippocampus.We induced LTP at the Schaffer collateral to CA1 (SC-CA1) synapse in acute hippocampal slice isolated from Rem2 WT or Rem2 cKO mice using high-frequency stimulation and found the loss of Rem2 was associated with increased levels of synaptic potentiation.Further, the effect of Rem2 on LTP was dependent on CaMKII.Finally, we demonstrated the ability of Rem2 and CaMKII to interact in dendritic spines in response to glutamate uncaging using two-photon excitation and fluorescence lifetime imaging microscopy and fluorescence resonance energy transfer (2pFLIM-FRET) in organotypic hippocampal slice preparations.

Animals
For LTP experiments, Rem2 flx/flx mice [40] were bred and housed in the animal facility at Brandeis University.For FLIM-FRET experiments C57BL/6N mice were purchased from Japan SLC, Inc. and were bred and housed in the animal facility in the National Institute of Natural Sciences.In both cases, animals were maintained with a 12-hour light-dark cycle.Food and water were available ad libitum.All animal procedures were approved by the Brandeis University Institutional Animal Care and Usage Committee or National Institute of Natural Sciences Animal Care and Use Committee, and all experiments were performed in accordance with relevant guidelines and regulations.

Immunohistochemistry in slices
Rem2 flx/flx mice were injected with AAV-GFP-Cre, AAV-Rem2-wt and AAV-Rem2 RR/GG at P27-P28 as described above.At 14 days post-injection (dpi), transcardial perfusion was performed with 4% paraformaldehyde/4% sucrose in 1X PBS solution.The brains were removed and fixed in 4% paraformaldehyde/4% sucrose for 24 h and then transferred to 30% sucrose for 24 h for storage.The brains were sectioned at 50 μm using a Leica VT1000S microtome and slices were suspended in PBS at 4˚C until being processed for immunohistochemistry. Brain sections containing hippocampus were blocked in PBS with 20% BSA and 0.1% Triton for two hours at room temperature prior to incubation with the primary antibody mouse anti-Rem2 (Santa Cruz, sc-514999) at a concentration of 1:100 overnight at 4˚C.The sections were then incubated in the secondary antibody (goat anti-mouse Alexa Fluor™ 546, Thermofisher A-21052 or goat anti-mouse Alexa Fluor™ 633, Thermofisher A-21052) at 1:500 for two hours at room temperature and mounted with Vectashield Hardset antifade mounting medium with DAPI (Vector Laboratories, H-1500).

Image acquisition and analysis
Images were acquired on a Zeiss LSM880 confocal microscope using the Plan-Apochromat 63x/1.40Oil DIC M27 objective.We acquired z-stacks (1.0 μm step size) of DAPI-positive and (where applicable) Cre-positive cell somas located within the pyramidal cell layer region of the CA1 subregion of the dorsal hippocampus.For each section, images including the pyramidal cell layer and striatum oriens were stitched together (~600 μm M/L and ~200 μm D/V) using the tile scan function; 2-3 sections were imaged per animal.Microscope settings for laser power, detector gain, and amplifier offset were initially optimized across multiple no-injection control samples and were kept constant within each experimental round.To minimize background noise, maximum intensity projection images were pre-processed using ImageJ in the following manner: In the channel containing anti-Rem2 staining, a small region of interest (~75x75 μm) was drawn in an area where neither cell bodies nor anti-Rem2 staining was visible, and the background in the anti-Rem2 channel was measured using the Analyze > Measure menu option in ImageJ.Then, the background was subtracted from the anti-Rem2 channel using the Process > Math > Subtract menu option, using the background value measured in the previous step.
Cell detection and counting and subsequent quantification of anti-Rem2 staining intensity was carried out using a semi-automatic pipeline in QuPath with maximum intensity projection images.Using the DAPI channel, a region of interest in the CA1 area of the hippocampus was drawn around the pyramidal cell layer in each stitched image.Within this region of interest, neuronal somas and nuclei were detected based on the DAPI-stained nucleus using the Analyze > Cell Detection menu option in QuPath, with software-recommended parameters.Rem2+ staining in the cell soma was detected by creating a classification filter, using the Classify > Object classification > Create single measurement classifier menu option in QuPath.A fixed pixel threshold value was empirically determined for mean fluorescence in the cytoplasm in the α-Rem2 channel.This threshold value was kept constant and applied to all images within each experimental round.Quantification of anti-Rem2 staining intensity in the dendritic arbors of CA1 pyramidal neurons was carried out using ImageJ with background subtracted maximum intensity projection images.A rectangular region of interest (~150x100 μm) was drawn in the striatum radiatum within the CA1 of the hippocampus in each stitched image.The average pixel intensity in the channel for anti-Rem2 staining was measured using the Analyze > Measure > Mean Gray Value menu option in ImageJ.

Field electrophysiology recordings
Brains were isolated from Rem2 flx/flx mice of both sexes 12-16dpi.The brain was rapidly dissected and placed in ice-chilled, oxygenated modified artificial cerebrospinal fluid (aCSF) comprising of (in mM): NaCl 124, KCl 3.7, NaHCO 3 24.6,CaCl 2 1, MgSO 4 4, D-glucose 10, KH 2 PO 4 1.2 saturated with 95% O 2 / 5% CO 2 .Parasagittal slices (400 μm thick) containing the hippocampal region were prepared in ice-chilled, oxygenated aCSF using a Leica VT1200 microtome.Following equilibration for at least an hour, slices were transferred to a brain slice interface chamber (model BSC2, Scientific Systems Design, Inc.); slices rested on filter paper at the interface of the perfusing solution (0.4-0.6 ml min -1 ) comprising of standard aCSF (in mM): NaCl 124, KCl 3.7, NaHCO 3 24.6,CaCl 2 2.5, MgSO 4 1, D-glucose 10, KH 2 PO 4 1.2 saturated with 95% O 2 / 5% CO 2 .Field recordings were obtained using a glass microelectrode (resistance * 2-4 MO) containing 3 M NaCl or standard aCSF, placed at the radiatum border.Responses were evoked by way of two stimulating electrodes placed within the Schaffer-collateral-commissural fibers.Baseline stimulation was every 30 s for each input with a 15 s interval between alternating inputs.Independence of inputs was assessed using a paired-pulse protocol.Stimulation intensity was adjusted such that the field evoked post-synaptic potential (fEPSP) slope was approximately 20-40% of that required to generate a visible population spike.LTP was induced by tetanic stimulation (100 Hz; 1 s) delivered 3 times at 5 minute intervals.The myristoylated CaMKIINtide, myr-CN27 (EMD Millipore, 208921) was bath applied where indicated.Recordings were filtered at 3-10 kHz using an Axoclamp 2A Amplifier (Molecular Devices), and collected for online analysis at a sampling rate of 20 kHz using WinLTP software [43].

Statistical analysis
Statistical analyses were performed using the R programming language [24].For all experiments we assessed the basic properties of samples (Q-Q plots, residual plots, distributions etc.) prior to running statistical tests with distribution assumptions.LTP experiments were analyzed using nonparametric bootstrapped multiple comparisons tests using custom-written code in R. The p values were corrected using the Sidak-Holm correction.Box plots were used to graphically represent summary data: the center line is the median, the edges of the box mark the interquartiles of the distribution and the lines extend to the limiting values of the sample distribution when outliers are excluded (defined as values further away from the median than 1.5 × interquartile range)

Results
Rem2 is expressed almost exclusively in the nervous system [25] and previous studies from our lab demonstrate that Rem2 is expressed in cortex and hippocampus [37,40].In addition, we previously showed that Rem2 mRNA expression is up-regulated in response to neuronal depolarization in cultured cortical neurons [39] as well as in response to sensory experience in visual cortex in the intact mouse brain [40].In hippocampus, Rem2 is expressed predominantly in the pyramidal cell layer of the CA1-CA3 subregions in young adult animals (Fig 1) [50,51].
Given the expression of Rem2 in pyramidal neurons in the CA1 area of the hippocampus, we sought to determine if Rem2 plays a role in CaMKII-dependent LTP at the CA3-CA1 Schaffer collateral synapse.To restrict Rem2 deletion to the CA1 region of the hippocampus, we injected Cre-expressing virus into that region in a Rem2 flx/flx mouse [40] by stereotaxis.Specifically, we injected an AAV9-hSyn-CreGFP virus by itself (Rem2 cKO) or we coinjected with a virus expressing either wild-type Rem2 (Rem2 cKO + wt-Rem2) or a mutant Rem2 which renders it unable to inhibit CaMKII kinase activity (Rem2 cKO + Rem2 RR/GG ) [24].
Given our previous results demonstrating endogenous Rem2 expression in the cell soma in cultured hippocampal neurons [52], we unexpectedly were unable to detect appreciable anti-Rem2 staining in the soma of the uninjected (i.e., wild-type) control animals (Fig 2B and 2C).However, we observed anti-Rem2 staining in the dendritic arbor of pyramidal neurons in the uninjected controls, suggesting that perhaps the Rem2 protein is more abundant in cell processes in P42 animals.To quantify this expression, we drew a region of interest in the striatum radiatum of the CA1 region (Fig 2D ) and measured the average pixel intensity per square micron in the anti-Rem2 channel.Our analysis showed that anti-Rem2 staining in the striatum radiatum was observable in both uninjected control and animals injected with Cre-GFP and a virus expressing wt-Rem2 at a similar level (Control 0.985 ± 0.373 per μm 2 x 10 −3 ; Rem2 cKO + wt-Rem2 1.193 ± 0.485 per μm 2 x 10 −3 ) (Fig 2E).Animals injected with Cre-GFP and a virus expressing Rem2 RR/GG showed a higher level of anti-Rem2 staining (Rem2 cKO + Rem2 RR/GG  3.529 ± 1.208 per μm 2 x 10 −3 ), while animals injected with Cre-GFP alone showed minimal anti-Rem2 staining (Rem2 cKO 0.348 ± 0.284 per μm 2 x 10 −3 ) confirming that the antibody detected Rem2 expression in all of our samples including uninjected controls (Fig 2E).
Next, we sought to determine if Rem2 deletion affects LTP.We injected 4-week-old Rem2 flx/flx mice with an AAV9-hSyn-GFP (control) or AAV9-hSyn-CreGFP virus (Rem2 cKO), or AAV9-hSyn-CreGFP together with a virus expressing wt-Rem2 (Rem2 cKO + wt-Rem2), or AAV9-hSyn-CreGFP together with a virus expressing Rem2 RR/GG (Rem2 cKO + Rem2 RR/GG ) into the CA1 region of the hippocampus.We subsequently performed field recordings from the CA1 area of acute hippocampal slices isolated from animals 14dpi.We induced LTP at the SC-CA1 synapse using high-frequency stimulation (comprising 3 tetanic stimulations of 100Hz for 1s delivered at intervals of 5 minutes) at t = 30 in control slices (Fig 3A and 3E; n = 10).Our results show that Rem2 deletion enhances potentiation compared to control slices (Fig 3B and 3E; n = 10), which lasted for at least 130 minutes post-tetanus.Additionally, while re-expression of wt-Rem2 rescued the enhanced LTP phenotype (Fig 3C and  3E; n = 10), expression of Rem2 RR/GG failed to rescue the enhanced LTP that is observed upon Rem2 knockout (Fig 3D and 3E; n = 10).From these data we conclude that Rem2 expressed in pyramidal neurons in CA1 normally acts as a brake on LTP at this synapse and that Rem2 inhibition of CaMKII is crucial to this process.
Given that Rem2 is a potent inhibitor of CaMKII kinase activity, we sought to determine if the enhanced LTP observed in the Rem2 cKO mice is due to increased CaMKII activity as opposed to an unknown synaptic function of Rem2 that is CaMKII-independent.To address this question, we injected 4-week-old Rem2 flx/flx mice with virus expressing GFP (control) or AAV9-hSyn-CreGFP (Rem2 cKO) and isolated acute hippocampal slices at 14dpi for hippocampal field recordings.In these recordings the CaMKII inhibitory peptide myr-CN27 [53] was bath-applied following a 30-minute baseline to block LTP induction (Fig 4).Following 50 minutes of myr-CN27 exposure, we delivered three 100Hz tetani with myr-CN27 still in bath.After delivery of the tetani myr-CN27 washout began at t = 80 min.After 60 minutes of myr-CN27 washout, we delivered another three 100Hz tetani and then recorded fEPSPs for another 60 minutes to ascertain if LTP was recovered.
Our results show that presence of myr-CN27 in the bath blocks the induction of LTP in both control (Fig 4A and 4C, n = 8) and Rem2 cKO conditions (Fig 4B and 4D, n = 9), strongly suggesting that the enhanced LTP observed in the Rem2 cKO animals is dependent on CaM-KII.The ability of this synapse to undergo LTP in both control and Rem2 cKO slices after myr-CN27 washout also supports this idea.However, we failed to observe enhanced LTP in the Rem2 cKO slices compared to the control upon myr-CN27 washout.Despite this fact, these data are consistent with the idea that enhanced LTP observed upon Rem2 knockout is CaMKII-dependent because if Rem2 were to signal through a CaMKII-independent pathway to inhibit LTP, myr-CN27 application in the Rem2 cKO condition should still have yielded some LTP induction and expression.
Our results suggest a model whereby Rem2 interacts with CaMKII at synapses to limit LTP.Although a previous study reports the co-localization of Rem2-GFP and mRuby-CaMKII in dendritic spines using diffraction-limited light microscopy [42], an analysis of Rem2-CaMKII dynamics at individual synapses undergoing plasticity has not been investigated.We hypothesized that CaMKII activation would cause a change in its association with Rem2 at the synapse.To address this question, we employed 2pFLIM which uses FRET-based sensors to measure protein-protein interactions in living cells [54].Cultured hippocampal slices isolated from P6-9 wild-type mouse pups were infected at DIV3 with an adeno-associated virus encoding Clover-CaMKIIα as the donor and AAV-DJ encoding mCherry-Rem2 as the acceptor.To induce structural LTP (sLTP), two-photon MNI-glutamate uncaging was carried out at DIV13-14 at 720 nm (30 trains, 0.5 Hz, 6 ms duration/pulse, 6 mW) at the tip of the spines (Fig 5A -5C) as described previously [55,56].Two-photon excitation at 1000 nm was used to excite Clover-control, (B) Rem2 cKO, (C) Rem2 cKO + Rem2-wt, and (D) and Rem2 cKO + Rem2 RR/GG ; (each n = 10).Shaded areas are the (bootstrapped) SEM.Single examples illustrate typical fEPSPs taken at the time points indicated (i, ii, iii, and iv).Vertical and horizontal scale lines indicate 0.5 mV and 5 ms, respectively.E) Summary box plots of normalized fEPSP slopes in the 4 conditions.Any significant effect is illustrated by *. https://doi.org/10.1371/journal.pone.0301063.g003

Discussion
CaMKII is a well-characterized, abundant protein kinase that mediates important functions in diverse cell types.In the nervous system, activation of CaMKII is both necessary and sufficient for the induction of NMDAR-dependent LTP, a biological correlate of learning and memory, at adult synapses in the hippocampus [23].The signaling pathways downstream of CaMKII which lead to synaptic potentiation are well-established [1,3,23].Autonomous CaMKII is not maximally active under basal conditions and its activity can be further stimulated ~5-fold by subsequent Ca 2+ /CaM binding [57].The role of endogenous, negative regulators of CaMKII activity, which in the nervous system could function to impede runaway potentiation, is not well understood.In this study we sought to determine if Rem2 functions to regulate LTP via its interaction with CaMKII.This line of inquiry was based upon a previous proteomic analysis that identified all four isozymes of CaMKII as interacting proteins with Rem2 and led us to the discovery that Rem2 is a direct, endogenous inhibitor of CaM-KIIα activity in vitro [24].
Here we provide the following evidence demonstrating that Rem2 acts as a post-synaptic brake on CaMKII-dependent LTP.First, we demonstrated that knockout of Rem2 in CA1 pyramidal neurons results in enhanced LTP at the SC-CA1 synapse.This effect was rescued by the reintroduction of wild-type Rem2 but not by a Rem2 mutant that failed to inhibit CaMKII in vitro.Next, we showed that the enhanced LTP observed upon Rem2 knockout is dependent on CaMKII as the specific CaMKII inhibitor myr-CN27 blocks this effect.Lastly, we observed an activity-dependent interaction between CaMKIIα and Rem2 at glutamatergic synapses in dendritic spines in the context of structural plasticity.
Interestingly, under the strong multiple stimulus condition employed in this study (Figs 3  and 4), LTP was thought to be saturated at the SC-CA1 synapse (i.e., potentiation could not be increased by further stimulation) presumably due to limitations on the components of LTP expression such as AMPAR recruitment to the synapse.Our novel and unexpected finding that Rem2 deletion enhances LTP strongly suggests that LTP was not in fact saturated in earlier studies because Rem2, previously unknown as a negative regulator of CaMKII-dependent LTP, was acting to restrict LTP at this synapse.LTP induction by CaMKII involves both a catalytic (i.e., phosphorylation at T286) and a structural, non-catalytic function (i.e., GluN2B binding) for CaMKII in LTP induction [20,21].Previous work from our lab demonstrated that Rem2 does not inhibit CaMKII autophosphorylation on T286 but does inhibit the autonomously active form of CaMKII [24].Therefore, what is the connection between Rem2 inhibition of CaMKII catalytic activity and LTP in vivo, given the fact that Rem2 RR/GG fails to rescue the enhanced LTP observed upon Rem2 knockout?We posit that once CaMKII is autonomously active and the catalytic domain is persistently exposed, the N-terminus of Rem2 accesses the substrate binding site, thereby inhibiting ongoing autonomous activity to exogenous substrates and GluN2B binding resulting in inhibition of CaMKII-dependent LTP.Note that while we previously demonstrated that Rem2 RR/GG fails to inhibit CaMKII in vitro [24], we do not yet know if or how these amino acid substitutions affect the ability of Rem2 to bind to CaMKII.One possibility is that the RR!GG substitutions alter the affinity of the Rem2 N-terminus for the substrate binding region on CaMKII, resulting in a lack of association between Rem2 and CaMKII, relieving any Rem2 interference in GluN2B binding to CaMKII.Thus, although our initial logic for testing the ability of Rem2 RR/GG to rescue enhanced LTP was based upon a purely catalytic model of CaMKII function in LTP, it is possible that Rem2 also inhibits the structural function of CaM-KII in LTP induction.
How might Rem2 interaction with CaMKII fit into our current understanding of CaMKII regulation and function at the synapse?Of course, Rem2 cannot entirely block CaMKII function or LTP would never be achieved.It could be that Rem2 is an alternative binding partner to GluN2B for CaMKII.In this scenario, Rem2:CaMKII could sequester a subset of active CaMKII from the synapse while inhibiting its catalytic activity, thereby blocking further LTP expression.In fact, our 2pFLIM-FRET data are consistent with the Rem2 sequestration of CaMKII out of the spine head upon binding (Fig 5).In parallel GluN2B:CaMKII could localize a distinct subset of active CaMKII molecules to the postsynaptic density, allowing for persistent LTP at the synapse.While the stoichiometry of Rem2:CaMKII and GluN2B:CaMKII has yet to be determined, CaMKII is approximately 100x more concentrated than GluN2B at the synapse [13], and given its abundance, CaMKII could also be present in excess of the Rem2 protein.A difference in relative abundance between Rem2 and CaMKII at synapses could also explain why we failed to observe enhanced LTP in the Rem2 cKO slices compared to control upon myr-CN27 washout (Fig 4).If myr-CN27 washout was incomplete, the remaining myr-CN27 bound to CaMKII could obscure the absence of Rem2 inhibition at the synapse by continuing to provide a moderate amount of CaMKII inhibition.
Previous studies from our lab demonstrated that Rem2 is also a CaMKII substrate and is phosphorylated by CaMKII on four conserved sites [38].We further speculate that Rem2 inhibition of CaMKII is overcome by the eventual phosphorylation of Rem2 by CaMKII.Phosphorylation of Rem2 by CaMKII could cause the dissociation of Rem2 from the CaMKII holoenzyme, thus serving as a negative feedback loop for Rem2 inhibition of CaMKII.This mechanism would effectively provide a time limit on Rem2 inhibition as potent, lasting inhibition could have deleterious effects on neuronal signaling.
Our finding that Rem2 deletion enhances LTP suggests the possibility that other negative regulators of CaMKII activity await discovery.In addition to Rem2, only the 79 amino acid CaMKIIN isoforms α and δ [58,59] and the postsynaptic density protein Densin-180 [60] are potential endogenous inhibitors of CaMKII in mammals.Although peptides based upon CaM-KIIN sequences are widely used to inhibit CaMKII and LTP in vitro and ex vivo, the function of CaMKIIN isoforms in regulating CaMKII-dependent synaptic plasticity is not well explored.However, CaMKIIN1 has been shown to play a role in long-term memory after retrieval in dorsal hippocampus [61].Interestingly, deletion of the Densin-180 gene interferes with localization of key proteins such as α-actinin to the PSD and impairs long-term depression (LTD) at the SC-CA1 synapse while having no effect on LTP [62].It is unclear which, if any, of these effects are dependent upon Densin-180 inhibition of CaMKII; it has been suggested that the role of Densin-180 in regulating voltage-gated calcium channel trafficking could underlie its role in synaptic plasticity [63].
Our results represent a paradigm shift in the understanding of LTP.Hundreds of structural, biochemical and functional studies inform the current model of CaMKII activation that underlies SC-CA1 LTP with little consideration of endogenous CaMKII inhibitors such as Rem2.Our finding that Rem2 deletion enhances LTP reveals a novel mechanism of regulation on CaMKII that limits LTP while shedding new insight into the role of Rem2 in the nervous system.

Fig 2 .
Fig 2. Viral-mediated conditional knockout of Rem2 and rescue with either wt-Rem2 or Rem2 RR/GG in hippocampus from P28 mice.A) Representative images from coronal sections of the dorsal CA1 from uninjected and virus-injected mice.Top, DAPI and anti-Rem2; middle, Cre-GFP; bottom, merged image.DAPI = blue, Cre-GFP = green, anti-Rem2 = red.B) Quantification of the percentage of neurons expressing indicated markers divided by DAPI-positive neurons for each experimental condition.C) Total number of neurons expressing indicated markers for each condition.D) Schematic for quantifying anti-Rem2 staining in the dendritic arbor of pyramidal neurons, from an uninjected

Fig 3 .
Fig 3. Conditional Rem2 deletion in the CA1 region of hippocampus enhances LTP at the SC-CA1 synapse.Mice from both sexes were bilaterally injected at P27-P29 with virus and acute hippocampal slices were prepared from injected mice for field recordings at P42-P46.Following a stable baseline period of at least 30 minutes, LTP was induced using three high-frequency stimulation bursts at 100 Hz.Comparisons were made at t = 170 min.A-D).Normalized fEPSP slope is plotted as a function of time for each of 4 conditions; both test and control inputs are illustrated: (A)

Fig 4 .
Fig 4. LTP observed in Rem2 cKO is CaMKII dependent.After a stable baseline (30 min) was obtained myr-CN27 (1 μM) was bath applied for 50 minutes then three 100 Hz tetani were delivered with myr-CN27 still in bath after which myr-CN27 was washed out for 60 minutes.Subsequently three 100Hz tetani were delivered and the recording was continued for 60 minutes.A, B) Normalized fEPSP slope as a function of time for control (A, n = 8) and Rem2 cKO (B, n = 9).Shaded areas are the (bootstrapped) SEM.Traces above the time plots represent the raw data comprising averages of time points within each experiment taken at the time points indicated (i, ii, etc.).As before, vertical and horizontal scale lines indicate 0.5 mV and 5 ms, respectively.C, D) Summary box plot to illustrate normalized fEPSP slope for control (A, n = 8) and Rem2 cKO (B, n = 9).Any significant effect is illustrated by *.There was no difference between control and Rem2 cKO animals for comparable conditions.https://doi.org/10.1371/journal.pone.0301063.g004

Fig 5 .
Fig 5. Rem2 interacts with CaMKII in a 2-photon FLIM-FRET assay.Representative fluorescence lifetime images of Clover-CaMKIIα and mCherry-Rem2 interaction (A) or Clover-CaMKIIα and mCherry interaction (D) during structural LTP induced by two-photon glutamate uncaging.The arrowhead indicates the stimulated spine.Warmer colors indicate shorter fluorescent lifetimes, therefore increased interaction between proteins.B) Time course of CaMKII and Rem2 interaction measured as a change in the fraction of Clover-CaMKIIα bound to mCherry-Rem2 in stimulated spines (blue, n = 7), the dendritic shaft beside the stimulated spines (orange, n = 7), and adjacent spines (black, n = 7).The right panel shows a 5-minute window of the imaging data from the left panel as indicated by green highlighting.Error bars = SEM.n = 5 neurons.The black bar indicates glutamate uncaging.C) Averaged time course of fluorescence change in the same experiments as in B for Clover-CaMKIIα and mCherry-Rem2 in stimulated spines (green for Clover-CaMKIIα, red for mCherry-Rem2, n = 7) and in adjacent spines (black, n = 7).The black bar indicates glutamate uncaging.Note that the difference in the time course of the change in fluorescence between Clover-CaMKIIα and mCherry-Rem2 suggests dynamic regulation of Rem2 localization.E) Time course of CaMKII and mCherry interaction measured as a change in the fraction of Clover-CaMKIIα bound to mCherry in stimulated spines (blue, n = 7), the dendritic shaft beside the stimulated spines (orange, n = 7), and adjacent spines (black, n = 11).The right panel shows a 5-minute window of the imaging data from the left panel as indicated by green highlighting.Error bars = SEM.n = 6 neurons.F) Averaged time course of fluorescence change in the same experiments as in E for Clover-CaMKIIα and mCherry, in stimulated spines (green for Clover-CaMKIIα, red for mCherry, n = 7) and in adjacent spines (black, n = 7).The black bar indicates glutamate uncaging.Note that the similarity in the time course of the change in fluorescence between Clover-CaMKIIα and mCherry reflects the fact that they are freely diffusing proteins[64].https://doi.org/10.1371/journal.pone.0301063.g005